Fig. 6
YTHDF2 mediates the decay of PARP1 mRNA to regulate NONO-TFE3 tRCC progression. a YTHDF1/2 was immunoprecipitated followed by qRT-PCR for assessing the association of PARP1 mRNA with YTHDF1/2 after overexpression or knockdown of TRAF3IP2-AS1. b Luciferase activity of PARP1-3′-UTR was measured after co-transfected with TRAF3IP3-AS1 and siYTHDF2. c Schematic illustration of MS2-RIP. d Abundance of m6A relative proteins among MS2-RIP with anti-GFP antibody from cells transfected with indicated plasmid was measured by Western blot. e The stability of PARP1 mRNA in cells co-transfected with indicated vectors or siRNAs after treatment with α-amanitin. f Schematic illustration of targeted RNA methylation system. g–h The mRNA (g) and protein (h) level of PARP1 were measured by qRT-PCR and Western blot after transfected with indicated gRNA. i–j The stability of PARP1 and GAPDH mRNA in cells transfected with indicated gRNA after treatment with α-amanitin. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001