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Fig. 8 | Journal of Hematology & Oncology

Fig. 8

From: Low expression of TRAF3IP2-AS1 promotes progression of NONO-TFE3 translocation renal cell carcinoma by stimulating N6-methyladenosine of PARP1 mRNA and downregulating PTEN

Fig. 8

TRAF3IP2-AS1 modulates the expression of PTEN through post-transcriptional regulation of miRNAs. a Schematic of the selection for the direct downstream target of TRAF3IP2-AS1. b–c The effect of multiple miRNAs expression on PTEN mRNA in UOK109 cells was analyzed by qRT-PCR after overexpression or knockdown of miRNAs, respectively. d RIP assays were performed using AGO2 antibody in UOK109 cells, and then, the enrichment of PTEN mRNA was detected by qRT-PCR. e HEK293T cells were co-transfected with miRNA mimics, respectively, and wild-type or mutant PTEN 3′-UTR luciferase reporter vector, and luciferase reporter activity was detected. f–h Schematic illustration of PTEN 3′-UTR wild type and mutation luciferase reporter vectors. i The mRNA levels of NONO-TFE3 and PTEN were detected after transfection with NONO-TFE3 plasmids or shTFE3 for 48 h. j–k The protein level of PTEN was detected after co-transfection with indicated vectors, siRNAs/ASOs or miRNAs inhibitor for 48 h. The data are presented as the mean ± SD, **P < 0.01, ***P < 0.001

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