Fig. 1
From: Targeting neddylation E2s: a novel therapeutic strategy in cancer
Overview of the neddylation pathway. a Impact of neddylation in cancers. b The process by which NEDD8 is conjugated to its substrates. NEDD8 is activated by an NAE in an ATP-dependent manner, transferred to E2 and then conjugated to a lysine residue of the substrate protein with the aid of E3 ligase. Then, NEDD8 is removed by a deneddylase from the substrate and recycled. c The C-terminal carboxylate of NEDD8 is conjugated to the active cysteine residue of the E2 enzyme in the catalysis of E1. The E2-NEDD8 conjugate reacts with a lysine residue on the substrate to form the NEDD8 linkage