Fig. 3

CLK1 promoted the migration and invasion ability of pancreatic cells in vitro and in vivo. a–d Transwell assays with stably transfected BxPC-3 (a, b) and PANC-1 (c, d) cells were performed. Representative images and quantification of the results are presented. e–h Wound-healing assays with stably transfected BxPC-3 (e, f) and PANC-1 (g, h) cells were performed. Representative images and quantification of wound closure are presented. i–l The epithelial–mesenchymal morphology of BxPC-3 (i) and PANC-1 (j) cells, scale bars for the images are 25 μm. And the protein expressions of E-cadherin, and Vimentin were analyzed, the quantification are presented on the right (k, l). m–t Control BxPC-3 cells or CLK-1-overexpressing BxPC-3 cells (m–p), or control PANC-1 cells, or CLK-1-knocked down PANC-1 cells (q–t) were injected into nude mice via tail vein, and tumor metastasis to lung was evaluated after 6 weeks. Representative bioluminescence images of mice are presented (m, o, q, s), and the number of pulmonary metastasis (n, r) was summarized. Mice survival analysis was further analyzed (p, t). Data are shown as mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, between the indicated groups