Fig. 2.

8-Cl-Ado and VEN synergize in inhibition of OXPHOS metabolism in AML. a + b Effects of combined treatment of 8-Cl-Ado and VEN on levels of OCR and ECAR in CD34+CD38− AML blasts. Cells were treated with control, 500 nM 8-Cl-Ado, 10 nM VEN or 8-Cl-Ado plus VEN for 24 h. Levels of OCR (a) and ECAR (b) were measured using the Seahorse XF cell energy phenotype test kit. c + d CD34+CD38− AML blast cells were treated for 48 h as described in a + b. c Effects of combined treatment of 8-Cl-Ado and VEN on levels of p21 and PCNA protein expression. Each lysate was immunoblotted with indicated antibodies. d Effects of combined treatment of 8-Cl-Ado and VEN on apoptosis levels. Left, genomic DNA was isolated and DNA fragmentation is shown. Right, apoptosis levels as measured by Annexin V/DAPI staining using flow cytometry