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Table 2 Primary cell-derived CAR-NK cells

From: Chimeric antigen receptor natural killer (CAR-NK) cell design and engineering for cancer therapy

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Reference: first author (journal, year)

Effector cell

Vehicle

Target

generation

Activation signal

retro

lenti

mRNA

plasmid

transposon

1

2

3

1

Imai (Blood 2005)

PB-NK

x

    

CD19

x

x

 

DAP10/CD137-CD3ζ/CD3ζ

2

Altvater (Clin Cancer Res 2009)

PB-NK

x

    

CD19, GD2

x

x

 

CD3ζ/2B4/2B4-CD3ζ

3

Li (Cancer Gene Therapy 2010)

PB-NK

  

x

  

CD19

 

x

 

CD137-CD3ζ

4

Shimasaki (Cytotherapy 2012)

PB-NK

  

x

  

CD19

 

x

 

CD137-CD3ζ

5

Boissel (Leukemia Lymphoma 2012)

PB-NK/CB-NK

 

x

x

  

GFP, CD19, CD20

x

  

CD3ζ

6

De Oliveira (Hum Gene Ther 2013)

HSC

 

2

   

CD19

x

x

 

CD3ζ/CD28-CD3ζ

7

Chu (Cancer Immunol Res 2015)

PB-NK

  

x

  

CD20

 

x

 

CD137-CD3ζ

8

Suerth (J Mol Med 2016)

PB-NK + NKL

a/g

3

   

CD19

  

x

CD28-CD137-CD3ζ

9

Oelsner (Int J Cancer 2016)

CIK

 

2

   

CD19

x

x

 

CD28-CD3ζ/CD3ζ

10

Chu (OncoImmunol 2017)

PB-NK

  

x

  

CD20

 

x

 

CD137-CD3ζ

11

Kailayangiri (OncoImmunol 2017)

PB-NK

x

    

GD2

 

x

x

CD137-CD3ζ/t2B4-CD3ζ/CD137-t2B4-CD3ζ/t2B4-CD137-CD3ζ

12

Kloess (Hum Gene Ther 2017)

PB-NK

a

    

CD123

  

x

CD28-CD137-CD3ζ

13

Liu (Leukemia 2018)

CB-NK

x

    

CD19

 

x

 

CD28-CD3ζ

14

Oei (Cancer Immunol Res 2018)

PB-NK

  

x

  

CD19

 

x

x

CD28-OX40-CD3ζ/OX40-CD3ζ/CD28-CD3ζ/CD137-CD3ζ

15

Yu (Mol Ther 2018)

PB-NK

 

2

   

GPC3

 

x

 

CD28-CD3ζ

16

Li (Cell Stem Cell 2018)

iPSC/NK-92

    

x

Mesothelin

x

x

x

2B4-CD3ζ/CD28-CD137-CD3ζ/DAP10-CD3ζ/CD137-CD3ζ/2B4-DAP12-CD3ζ/2B4-DAP10-CD3ζ/CD137-2B4-CD3ζ/CD3ζ

17

Kloess (Hum Gene Ther 2019)

PB-NK/NK-92

a

    

CD123

  

x

CD28-CD137-CD3ζ

18

Oberschmidt (Hum Gene Ther Meth 2019)

PB-NK

a

    

CD123

  

x

CD28-CD137-CD3ζ

19

Colamartino (Front Immunol 2019)

PB-NK

 

2

   

CD19, CD22

  

x

CD28-CD137-CD3ζ

20

Ingegnere (Front Immunol 2019)

PB-NK/NK-92

   

x

 

CD19

x

x

 

CD3ζ/CD137-CD3ζ

21

Herrera (Sci Rep 2019)

CB-NK/PB-NK

 

3

   

CD19

 

x

 

CD137-CD3ζ

22

Bari (Front Immunol 2019)

PB-NK

 

3

   

CD19

 

x

 

CD137-CD3ζ

23

Wang (Blood Advance 2020)

PB-NK

x

    

BCMA, CD123

x

  

CD3ζ

24

Liu (NEJM 2020)

CB-NK

x

    

CD19

 

x

 

CD28-CD3ζ

25

Muller (Front Immunol 2020)

PB-NK

a

3

   

CD19

 

x

 

CD28-CD3ζ

26

Quintarelli (Leukemia 2020)

PB-NK

x

    

CD19

 

x

 

CD137-CD3ζ

27

Ueda (Cancer Sci 2020)

iPSC

 

x

   

GPC3

  

x

CD28-CD137-CD3ζ

28

Liu (Cell Proliferation 2020)

PB-NK

 

2

   

EGFR

 

x

 

CD137-CD3ζ

29

Gang (Blood 2020)

PB-NK

 

3

   

CD19

 

x

 

CD137-CD3ζ

30

Yang (Mol Ther Methods Clin Dev 2020)

PB-NK

x

    

CD19

 

x

 

CD137-CD3ζ

31

Wilk (Blood Advances 2020)

PB-NK

  

x

  

CD19

–

–

  

32

Daher (Blood 2020)

CB-NK

x

    

CD19

 

x

 

CD28-CD3ζ

33

Jamali (Front Immunol 2020)

PB-NK/NK-92/NKL

 

3

   

CD19

 

x

 

CD137-CD3ζ

34

Gurney (Haematologica 2020)

PB-NK

  

x

  

CD38

 

x

 

CD28-CD3ζ

35

Tseng (Nat Commun 2020)

NK-92MI, PB-NK

x

x

   

CD147, GPC3

  

x

CD28-CD137-CD3ζ

  1. Numbers in the "Vehicle" column refer to the lentivirus generation. For retroviruses: a = alpha, g = gamma. – denotes information not provided in the study. Other abbreviations: gen. CAR generation, Lenti lentivirus, Retro retrovirus, Transp transposon, PB-NK peripheral blood-derived NK cells, CB-NK cord blood-derived NK cells, CIK Cytokine-induced killer cells
  2. There is an interactive and extended version of this table available online, also providing regular updates: http://www.carnkreview.com