Table 5 NK cell sources
From: Chimeric antigen receptor natural killer (CAR-NK) cell design and engineering for cancer therapy
NK cell source | Advantages | Disadvantages |
|---|---|---|
Peripheral blood NK cell | Commonly used source | Low transduction efficiency |
Mature NK cells | Expansion induces telomere shortening and reduced cytotoxicity | |
Easily expanded ex vivo | Heterogeneity | |
Cord blood-derived NK | High proliferative capacity | Low expression of natural cytotoxicity receptors |
Safety and persistence demonstrated in CAR-NK trials | Heterogeneity | |
Stem cell-derived NK cells | Off-the-shelf product | Potentially immunogenic |
Homogeneity | Potential of malignant transformation | |
Less cytokine release but still high killing capacity | Â | |
NK-92 and other NK cell lines | Off-the-shelf product | Tumor-derived cells, requires irradiation |
Stable expression of CAR on the surface | Irradiated CAR-NK-92 cells are not sustained in vivo | |
Irradiated CAR-NK-92 cell already used in clinical trials | Not capable of ADCC (without additional modifications) |