Fig. 5

Blockade of PVRIG reverses exhaustion of tumor-infiltrating NK cells in tumor-bearing mice. Mice were injected with PBS, anti-PVRIG mAb or isotype-matched control mAb (rat IgG) intraperitoneally (i.p.) at various time points after injection of 5 × 10⁴ MC38 tumor cells subcutaneously (s.c.) on day 0 and killed on day 28 after challenge as described in Fig. 4a (n = 8 per group). a Representative histograms (top) and quantification (bottom) of PD-1 and CD96 expression in tumor-infiltrating NK cells. b Representative histograms (top) and quantification (bottom) of NKG2D and TRAIL expression in tumor-infiltrating NK cells. c Representative histograms (top) and quantification (bottom) of CD107a and Granzyme B (GzmB) expression in tumor-infiltrating NK cells. d Representative histograms (top) and quantification (bottom) of IFN-γ synthesis in tumor-infiltrating NK cells. e Absolute number of tumor-infiltrating NK cells in mice treated with rat IgG or anti-PVRIG mAb. Each symbol represents an individual mouse (n = 8 (a–d) or n = 5 (e) per group). Data were representative of two independent experiments. Error bars represent means ± s.e.m. Statistical significance was determined using unpaired two-tailed t test. *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001