Fig. 5

NOTCH1-inhibition synergizes with nuclear export inhibition in vitro. a Growth curves for HSB-2, MOLT4, RPMI-8402, ALL-SIL, DND-41, HPB-ALL, JURKAT and LOUCY cell lines in the presence of DMSO, KPT-8602 50 nM, MRK-560 0.1 µM or both inhibitors. Data are shown as mean of biological triplicates. Standard deviation is depicted only when larger than the used symbol. b Dose–response curve for KPT-8602 for DND-41, RPMI-8402, ALL-SIL and LOUCY. Data represent the mean of biological triplicates for any given concentration. c Bar charts showing cell numbers for DND-41, RPMI-8402 and ALL-SIL relative to control for different concentrations of KPT-8602 after 7-day pretreatment with DMSO, MRK-560 0.1 or 1 µM. Data are shown as mean ± standard deviation of biological triplicates. Significance was calculated using one-way ANOVA with Bonferroni’s multiple comparisons correction. d Annexin V-PI staining in DND41 and ALLSIL cells after 12 days of treatment with DMSO, KPT-8602 (50 nM), MRK-560 (1 µM) or combination. Data represent mean ± standard deviation for biological triplicates. Data were analyzed using one-way ANOVA with Bonferroni multiple comparisons correction. e Combination index plots according to Chou-Talalay. Each dot represents a concentration combination. ns = not significant (p > 0.05), *p < 0.05, **p < 0.01, ****p < 0.0001