Fig. 6

Combination of MRK-560 and KPT-8602 reduces leukemia burden in vivo. a Schematic representation of the experiment. b Percentage of hCD45⁺ cells in the blood as assessed by flow cytometry analysis of hCD45-APC after facial vein blood collection over time (left) and at day 15 (right). Each dot represents a single animal; mean and standard deviation are shown at day 15. Data were analyzed by one-way ANOVA; Bonferroni correction for multiple comparisons testing was applied. c Bioluminescence imaging results for all mice, measured as maximum total flux (photons/second). Evolution over time (top) and analysis at day 14 (bottom, left) of the experiment are shown. Each dot represents a single animal; bars represent mean and standard deviation. Fold change in maximum total flux between end and start of treatment was calculated for each mouse (bottom, middle). Data were analyzed by one-way ANOVA; Bonferroni correction for multiple comparisons testing was applied. Normalized luminescence imaging overlay for all mice at days 0, 7, and day 14 is shown on the right panel. d Spleen weight and hCD45 staining results for spleen and bone marrow on day 15. Each dot represents an animal; mean and standard deviation are shown. Data were analyzed by one-way ANOVA, and Bonferroni correction for multiple comparisons testing was applied. e Kaplan–Meier plot showing survival for the remaining 5 mice in every treatment group. Gray area indicates treatment period. f Body weight change during treatment for each animal (top) and quantification of number of goblet cells per millimeter of villus (bottom) (n = 3). Mean and standard deviation are shown. g Representative images of PAS staining from mice treated with vehicle, KPT-8602, MRK-560 or combination for 14 days. Scale bar, 200 µm. ns = not significant (p > 0.05), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001