Fig. 1

Clinical response to anti-PD-1 therapy and biological biomarker detection. a Anti-PD-1 treatment response of each patient and duration of response following anti-PD-1 salvage therapy. UPN1 had 100% decrease in baseline tumor burden and another new lesion occurred, and evaluated as disease progression after 10 months. CR, complete response; PR, partial response; SD, stable disease; PD, progressive disease. b Percentage change of tumor burden after anti-PD-1 therapy compared to baseline level prior to anti-PD-1. c, d Percentage of PD-1⁺CD3⁺ cells in tumor-infiltrated CD3⁺ cells (c) or PD-L1⁺ cells in CD19 and CD20 tumor cells (d) from all five patients before CART19/20 cell infusion and after disease progression or relapse post-CART therapy. Responses to anti-PD-1 salvage therapy were shown. e PET/CT imaging for UPN4 before anti-PD-1 and 2 months after anti-PD-1 therapy. f Percentage of IFN-γ⁺CD8⁺ cells, IFN-γ⁺CD4⁺ cells, PD-1⁺CD8⁺ cells and PD-1⁺CD4⁺ cells in peripheral blood prior to anti-PD-1 and 14 days after anti-PD-1 treatment in UPN4 by flow cytometry. g The absolute number of CART19/20 cells in UPN4 by PCR. h Serum cytokine levels in UPN4 following PD-1 blockade therapy. UPN, unique patient number