Fig. 2

LCAT3 is upregulated by METTL3 through m6A modification. A METTL3 is upregulated in LUAD. Boxplot showing the relative expression of METTL3 in lung tumors (n = 480) and adjacent normal tissues (n = 56) in TCGA LUAD cohort. METTL3 expression was quantified by FPKM in the RNA-seq data. B Western blot assays to detect METTL3 knockout efficiency in A549 and Calu1 cells. C, D qRT-PCR analysis of LCAT3 expression in sg-lacZ and sg-METTL3 lung cancer cells. E MeRIP-seq of sgLacZ (top) and sgMETTL3 (bottom) A549 cells showed that depletion of METTL3 reduces the m6A modification on LCAT3. Red line represents input group while blue line represents IP group. F MeRIP-RT-qPCR confirmed the change in m6A levels in METTL3-knockout A549 cells. G, H Half-life of LCAT3 in sg-lacZ and sg-METTL3 lung cancer cells treated with 5 μg/mL Actinomycin D