Fig. 8

LCAT3 recruits FUBP1 to activate MYC expression. A qRT-PCR showing the interaction of FUBP1 with the MYC FUSE sequence in A549 cells from the ChIP assay. IgG was used as negative control. B, C ChIP analysis showing that the depletion of LCAT3 decreases the enrichment of FUBP1 at MYC FUSE sequence both in A549 (B) and Calu1 cells (C). D A schematic diagram of luciferase report assay. E MYC FUSE and promoter sequences (+ 33 to − 1896 bp) were constructed into pGL3 plasmid and subjected to luciferase reporter assays in either LCAT3 or FUBP1-silenced 293T cells. F FUSE-deficient MYC promoter sequences (+ 33 to − 1647 bp) were constructed into pGL3 plasmid and subjected to luciferase reporter assays in either LCAT3 or FUBP1-silenced 293T cells. G Proposed model for the LCAT3-FUBP1-MYC axis regulating the proliferation, migration, and invasion of lung cancer cells