Fig. 3

YTHDF2 may active NF-κB through suppressing its negative regulators. A KEGG pathways of the genes that positively correlated with YTHDF2 expression in gliomas from four datasets. The blue point shows the − log P value, and the red box shows the gene counts in each terms. B, C GSEA of hallmarks genes with increased expression in YTHDF2 high expression groups. D Immunostaining image showing NF-κB (p65) protein expression and localization in N33 cells with or without YTHDF2 siRNA. Bar = 10 μm. E Venn diagram shows the overlap genes whose expression is negatively correlated with YTHDF2 expression in primary gliomas form different datasets and negatively regulate NF-κB signaling. F Correlation of YTHDF2 expression and the 5 selected genes in gliomas from CGGA microarray and TCGA RNA-seq datasets