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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: YTHDF2 facilitates UBXN1 mRNA decay by recognizing METTL3-mediated m6A modification to activate NF-κB and promote the malignant progression of glioma

Fig. 5

YTHDF2 can facilitate UBXN1 mRNA decay through recognizing m6A modification on UBXN1 mRNA that mediated by METTL3. A MeRIP-sequencing data of UBXN1 in U87 cells with or without shRNA-mediated METTL3 knockdown (METTL3 KD). B MeRIP-PCR data shows the relative quantity of UBXN1 mRNA immunoprecipitated by the m6A antibody (m6A-IP) and IgG in cells with or without METTL3 KD. **P < 0.01; ***P < 0.001; **P < 0.0001. C, D RIP-PCR showing the content of UBXN1 mRNA immunoprecipitated by METTL3 and YTHDF2 antibodies. IgG antibodies were used as negative control. ****P < 0.0001. E RIP-qPCR showing the content of UBNX1 immunoprecipitated by YTHDF2 antibodies in U87 cells with or without METTL3 shRNA. F The stability of UBXN1 mRNA in U87 cells with or without YTHDF2 siRNA. G The stability of UBXN1 mRNA in U87 cells with or without YTHDF2 overexpression (OE). H The expression levels of UBXN1 mRNA in U87 cells with or without METTL3 shRNA. I The expression levels of UBXN1 mRNA in U87 cells with or without METTL3 OE. J The stability of UBXN1 mRNA in U87 cells with or without METTL3 shRNA. K The stability of UBXN1 mRNA in U87 cells with or without METTL3 OE. L METTL3, UBXN1, pp65, and p65 protein expression in U87 cells with or without YTHDF2 OE

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Journal of Hematology & Oncology

ISSN: 1756-8722

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