Fig. 7
YTHDF2 and METTL3 promotes tumor growth and leads to a worse prognosis in an orthotopic xenograft model. A Representative tumor bioluminescence images of mice at 10, 20, and 30 days after tumor implantation in an orthotopic xenograft model generated by U87 cells transfected with an empty vector, YTHDF2 OE, and METTL3 OE vector. The representative magnetic resonance imaging of mice at 21 days after tumor implantation were also shown in the right panel. B The bioluminescence intensity of mice at 10, 20, and 30 days after tumor implantation. C Kaplan–Meier survival curve of nude mice with tumors. D Representative images of H&E and immunohistochemical staining for YTHDF2, UBXN1, and p65 in the tumors of nude mice. Bar = 3 mm in the left panel. Bar = 10 μm in the right panel. E The mechanistic scheme by which YTHDF2 facilitates UBXN1 mRNA decay via recognizing m6A modification that mediated by METTL3 to activate NF-κB and promote tumor malignancy