From: Therapeutic roles of mesenchymal stem cell-derived extracellular vesicles in cancer
Isolation method | Principle | Advantages | Disadvantages | References |
---|---|---|---|---|
Differential ultracentrifugation | Based on differential centrifugation | Most commonly used and well established Simple Economical Relatively high yield | Low purity Low upscale potential | |
Density gradient ultracentrifugation | Based on density gradient of solutions | Relatively high purity Maintain EV integrity | Time-consuming Lower yield Low upscale potential | |
Size exclusion chromatography | Based on particle size | Economical Relatively high purity Maintain EV integrity High upscale potential | Time-consuming Lower yield Contamination | [32] |
Tangential flow filtration | Based on particle size | High yield High purity High time-efficiency High upscale potential | Complicated equipment Difficult operation Limited understanding | |
Affinity capture | Based on interaction of capture molecule with EV antigen | High purity Specific separation | Low yield Costly Separate targeted proteins only |