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Table 2 Diagnostic significance of sEV-derived ncRNAs in PDAC

From: Small extracellular vesicle non-coding RNAs in pancreatic cancer: molecular mechanisms and clinical implications

Reference Sample sEV isolation Normalization ncRNA (expression in PC) Patients enrolled Diagnostic accuracy (AUC) (PC vs. non-PC if not stated otherwise)
(↑) (↓)
Que et al. [142] Serum UC RNU6B miR-17-5p
miR-21
22 PDAC, 6 BPT, 7 AC, 6 CP, 8 HC 0.887 (miR-17-5p)
0.897 (miR-21)
Madhavan et al. [156] Serum DGUC RNU6, SNORD43
18S & 5S rRNA
miRs 1246, 4644, 3976, 4306 112 PDAC, 11 other PC, 22 BPT, 23 CP, 18 HC Not assessed
Machida et al. [158] Saliva TEI RNU6 miR-1246
miR-4644
12 PBC (thereof 9 PDAC), 13 HC 0.814 (miR-1246), 0.763 (miR-4644)
0.833 (miRs 1246 + 4644)
Lai et al. [146] Plasma UC miR-425-5p miRs 10b, 20a, 21, 30c, 106b, 181a miRs let7a, 122 29 PDAC, 11 CP, 6 HC 1.00 (miRs 10b, 21, 30c, 181a, let7a)
0.99 (miR-122), 0.95 (miR-20a), 0.85 (miR-106b)
Chen et al. [229] Serum UC 5S rRNA miR-23b-3p 16 PDAC, 20 HC 1.00 (miR-23-3p)
Xu et al. [157] Plasma EQ cel-miR-54 miR-1246
miR-196a
miR-196b (n.s.)
15 PC (UICC I-IIa)
15 HC
0.73 (miR-1246)
0.81 (miR-196a)
0.71 (miR-196b)
Goto et al. [145] Serum EQ not specified miRs 21, 191, 451a 32 PC, 22 HC 0.826 (miR-21), 0.788 (miR-191)
0.759 (miR-451a)
Zhou et al. [152] Plasma EQ miR-103a miR-122-5p,
miR-193b-3p
miR-221-3p 31 PC, 37 HC 0.772 (miR-122-5p), 0.651 (miR-193b-3p)
0.849 (miRs 122-5p + 193b-3p)
Kitagawa et al. [162] Plasma not specified not specified SNORA74A, SNORA25, SNORA22, SNORA14B, SNORD22 27 PDAC, 13 HC 0.909 (SNORA74A)
0.903 (SNORA25)
0.883 (SNORA22)
0.875 (SNORA14B)
0.862 (SNORD22)
Nakamura et al. [160] PJ UC miR-16 miR-21
miR-155
27 PDAC, 8 CP 0.83 (miR-21)
0.89 (miR-155)
0.91 (miR-21 + miR-155)
Zou et al. [230] Serum EQ cel-miR-39 miRs 19a, 19b, 192 32 PC, 32 HC Not assessed
Shang et al. [204] Plasma UC RNU6 miR-1231 32 PC, 20 HC Not assessed
Sun et al. [76] Plasma UC not specified miR-3607-3p 40 PC, 20 HC Not assessed
Takahashi et al. [55] Serum EQ RNU6B lncHULC 20 PDAC, 22 IPMN,
21 HC
0.92
Reese et al. [155] Serum UC cel-miR-39 miRs 200b, 200c 56 PDAC, 11 CP, 22 HC 0.77 (miR-200b TSE) 0.68 (miR-200c TSE)
0.97 (4-miR-panel + CA.19-9)
Pu et al. [151] Plasma TEI n/a * miR-10b, miR-21 36 PC, 65 HC 0.6543 (miR-10b)
0.7171 (miR-21)
0.791 (miR-10b + miR-21)
Yoshizawa et al. [159] Urine EQ miR-8069 ratio of miRs 3940-5p/8069 43 PDAC, 12 CP, 25 HC 0.732
Yang et al. [167] Plasma TENPO not specified miR-409 57 PDAC, 49 HC, 12 CP, 3 IPMN, 12 BPT, 3 others 0.95 (5-marker-panel including miR-409,
2 mRNAs, ccfDNA, and CA.19-9)
Flammang et al. [231] Serum UC cel-miR-39 miR-192-5p 44 PDAC, 11 CP, 12 HC 0.83 (PDAC vs. HC)
0.54 (PDAC vs. CP)
Wu et al. [144] Serum EQ cel-miR-39 miR-21, miR-210 30 PC, 10 CP 0.83 (miR-21), 0.85 (miR-210)
0.90 (miR-21 + miR-210)
0.9 (miR-21 + CA-19-9)
0.9 (miR-210 + CA.19.9)
Guo et al. [116] Serum EQ β-actin lncUCA1 46 PC, 16 HC 0.7813
Zhou et al. [150] Plasma MC CD81 miRs 10b, 21, 451a 30 PC, 10 CP 0.875 (miR-10b)
0.939 (miR-21)
0.930 (miR-451a)
1.00 (miRs 10b + 21 + 451a + EphA2)
Cao et al. [107] Plasma TEI cel-miR-39 miR-616
miR-4465
50 PC, 30 HC Not assessed
Wang et al. [232] Serum EEM RNU6B miR-1226 17 PDAC, 12 BPD 0.74 (PDAC vs. BPD)
Shao et al. [154] Serum EQ cel-miR-39 miR-483-3p 63 PDAC, 22 HC 0.69 (sEV miR-483-3p)
0.84 (serum + sEV miR-483-3p)
Han et al. [115] Plasma EQ RNU6 lncCCAT1 93 PC, 93 HC not assessed
  1. AC, ampullary carcinoma; AUC, area under the receiver operating characteristic curve; BPT, benign pancreatic tumor; cel, caenorhabditis elegans; ccfDNA, circulating cell-free DNA; CP, chronic pancreatitis; DGUC, density gradient ultracentrifugation; EEM, ExoEasy Maxi Kit (Qiagen); EQ, ExoQuick Exosome Isolation Kit (System Biosciences); HC, healthy control; MC, microfluidic chip; mRNA, messenger RNA; other PC, non-PDAC pancreatic cancer; n.s., not significant; PanNET, pancreatic neuroendocrine tumor; PBC, pancreatobiliary cancer; PC, pancreatic cancer (all entities); PDAC, pancreatic ductal adenocarcinoma; PJ, pancreatic juice; TEI, Total Exosome Isolation Kit (ThermoFisher); TENPO, track etched magnetic nanopore device; UC, ultracentrifugation
  2. *Pu et al. utilized a tethered cationic lipoplex nanoparticles biochip for direct and absolute quantification of sEV microRNA, obviating the need for normalization