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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: SIRPα-αCD123 fusion antibodies targeting CD123 in conjunction with CD47 blockade enhance the clearance of AML-initiating cells

Fig. 5

SIRPα-αCD123 fusion antibodies enhance NK cell-mediated lysis of MOLM-13 and PDX AML cells. A NK cell-mediated dose-dependent lysis of MOLM-13 cells after 4 h at an E:T ratio of 5:1 measured by calcein AM release. Mean values ± SEM for n = 6 different NK cell donors are shown. EC50 values were calculated where possible. B Expression of CD123, CD33 and CD47 in primary AML samples assessed by flow cytometry. C, D NK cell-mediated lysis of AML cells in long-term culture at a 10 nM antibody concentration after 20 h at an E:T ratio of 5:1 measured by flow cytometry. The results from n = 7 different AML patient samples are represented as different symbols, and their mean values are shown. E Percentage of CD69+ cells measured by flow cytometry. F NK cell-mediated lysis of PDX cells at 100 nM antibody concentration after 20 h at an E:T ratio of 5:1 measured by flow cytometry. The results from n = 3 different AML patient samples are represented as different symbols, and their mean values are shown. Statistical differences were determined by one-way ANOVA using Holm-Sidak's post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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