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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: SIRPα-αCD123 fusion antibodies targeting CD123 in conjunction with CD47 blockade enhance the clearance of AML-initiating cells

Fig. 6

Ex vivo treatment with 2 × SIRPα-αCD123 prevents outgrowth of AML-491 PDX cells in vivo. A Experimental design of the engraftment assay. Residual AML cell from ADCC in (Additional file 1: Figure 3A, B) was FACS sorted, and equal volumes were injected intravenously into NSG mice. Mice were monitored using in vivo BLI. B, C AML burden in individual mice of the 10 × LIC and 100 × LIC groups measured by BLI. The dotted line indicates a total flux of 5 × 107 photons/s as the cut-off for evaluating positive AML engraftment. Statistical analysis was performed using Chi-squared test. Representative images of mice injected with 10 × LIC (D) and 100 × LIC (E) on day (d) 62. Mice that died in narcosis during imaging (d.i.n.) were counted as positive if the last imaging signal showed positive engraftment (p. e.) or were excluded from analysis (ex.) if not engrafted. F Kaplan–Meier curve of AML-491 engraftment analysed by BLI. (G) Kaplan–Meier curves showing survival of mice. Statistical significance was calculated using the log-rank test. *p < 0.05, **p < 0.01, not significant (ns)

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