Fig. 1

The novel metabolism-related lncROPM is highly expressed in BCSCs. A Cluster heat maps of differentially expressed lncRNAs (fold-change > 2, p-value < 0.05) between non-BCSCs (CD44^−/lowCD24⁺ cells) and BCSCs (CD44⁺CD24^−/low mammospheres) from MCF7 cells. B Heat maps of metabolic pathway-related lncRNAs. C qRT-PCR analysis of lncROPM expression in non-CSCs and CSCs from various breast cancer cells. D Localization of lncROPM in MCF7 CD44⁺CD24^−/low cells and CSCs was detected by RNA-FISH analysis (Original magnification, × 200, Scale bar, 50 μm). 18S and U6 served as a positive control for cytoplasmic and nuclear fractions, respectively. The nuclei were stained with DAPI. Fluorescent staining: DAPI (blue), lncROPM (red), 18S (red), U6 (red). E Subcellular localization of lncROPM in MCF7 CD44⁺CD24^−/low cells and CSCs was measured by qRT-PCR analysis. GAPDH and U6 mRNA were used as a control for cytoplasmic and nuclear transcripts, respectively. The data are presented as the mean ± SD, *p < 0.05, **p < 0.01