Skip to main content
Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties

Fig. 5

LncROPM binds to the 3'UTR of PLA2G16 mRNA to enhance PLA2G16 mRNA stability. A, B qRT-PCR analysis of PLA2G16 pre-mRNA (intron1, intron2, intron3) and mature mRNA (3'-UTR, CDS and 5'-UTR) expression in MCF7 BCSCs (A) and BT549 CSCs (B) transfected with shCtrl, shROPM. C RNA pull-down assays were applied to detect the relative enrichment of PLA2G16 3'-UTR in MCF7 and BT549 BCSCs by using the indicated biotinylated probes, and the mRNA level was assessed by using qRT-PCR. D PLA2G16 3'-UTR was divided into two parts as shown in diagram. E, F The luciferase activity of vectors containing different regions of PLA2G16 3'-UTR was determined in BCSCs (E) transfected with shCtrl or shROPM, and in non-BCSCs (F) with LEV and LV-ROPM. G Luciferase activity was measured in 293 T cells co-transfected with lncROPM different fragment plasmid and luciferase reporter containing PLA2G16-3'-UTR-1. H, I BCSCs with shCtrl or shROPM (H) and non-BCSCs with LEV or LV-ROPM (I) derived from MCF7 and Hs578T were treated with actinomycin D (2 µg/mL) for the indicated times, respectively. qRT-PCR was used to detect the mRNA half-life of PLA2G16. Data were presented as the mean ± SD, *p < 0.05, **p < 0.01

Back to article page