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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: N6-methyladenosine reader IMP2 stabilizes the ZFAS1/OLA1 axis and activates the Warburg effect: implication in colorectal cancer

Fig. 3

IMP2 mediates biological characteristics by regulating ZFAS1 expression in CRC cells. a Flow-chart for detecting CRC phenotypes after single intervention with IMP2 or simultaneous intervention with IMP2 and ZFAS1. b MTT assay was performed to identify the cell viability upon IMP2 silencing or overexpressing in HCT116 cells. c CFA displaying the colony-forming abilities in both HCT116 and SW620 cells after ectopic or knockdown IMP2. d The migration ability was determined after ectopic or knockdown IMP2 in HCT116 and SW620 cells. e The percentage (%) of cell apoptosis was detected upon IMP2 overexpressing or silencing in HCT116 and SW620 cells by Flow cytometry. f Rescue experiments detecting the cell viability treated by co-transfected IMP2 silencing and ZFAS1 overexpression/IMP2 overexpression and ZFAS1 silencing vectors in HCT116 cells assayed by MTT assay. g, h, i Rescue experiments detecting the colony-forming abilities, migration capacity, and cellular apoptotic rates after co-transfected with IMP2 silencing and ZFAS1 overexpression or IMP2 overexpression and ZFAS1 silencing vectors in both HCT116 and SW620 cells assayed by CFA method, trans-well assays, flow cytometry, respectively. Data were shown as mean ± s.d. of at least three independent experiments. Data were shown as mean ± s.d. of at least three independent experiments. *P < 0.05; **P < 0.01; *** P < 0.001; **** P < 0.0001 and ns no significance

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