Skip to main content
Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Circular RNA circHERC4 as a novel oncogenic driver to promote tumor metastasis via the miR-556-5p/CTBP2/E-cadherin axis in colorectal cancer

Fig. 1

Identification and characterization of circHERC4 in CRC cells. a Pattern diagram of the method to screen circHERC4. b Explanation of the illustrated genomic loci of HERC4, and the verification strategy for the circular exon 4–8 (circHERC4). Sanger sequencing following PCR was used to show the “head-to-tail” splicing of circHERC4. c Relative RNA level of circHERC4 and linear HERC4 treated with RNase R, ***, P < 0.001. d Relative RNA level of circHERC4 and linear HERC4 in different time point treated with actinomycin D, ***, P < 0.001. e Nuclear and cytoplasmic fractions were isolated. CircHERC4 was mainly localized in cytoplasm. Malat 1 was substantially expressed in nucleus and Mt-COI was mostly contained in cytoplasm, which served as cytoplasmic and nuclear RNA marker

Back to article page