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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: Circular RNA circHERC4 as a novel oncogenic driver to promote tumor metastasis via the miR-556-5p/CTBP2/E-cadherin axis in colorectal cancer

Fig. 4

CircHERC4 accelerated CRC cell proliferation, migration and invasion by interacting with miR-556-5p. a Luciferase report experiment was used to pre-screen microRNAs that could bind to circHERC4. b RNA pull-down assays demonstrated that miR-556-5p was significantly enriched in the circHERC4 probe compared to NC probe, **, P < 0.01, ***, P < 0.001. c Expression of miR-556-5p was significantly downregulated in CRC tissues compared to matched adjacent normal tissues, ***, P < 0.001. d Expression of miR-556-5p in CRC tissues with lymphatic metastasis was significantly lower than those CRC tissues without lymph node metastasis, **, P < 0.01. e Relative RNA level of miR-556-5p was higher in the CRC tissues without distant metastasis compared to the CRC tissues with distant metastasis, *, P < 0.05. f CCK8 assays demonstrated that overexpression of miR-556-5p could inhibited the viabilities of DLD-1 and HCT116 cells, ***, P < 0.001. g Colony formation assays showed that overexpression of miR-556-5p repressed the formation of colonies in DLD-1 and HCT116 cells, ***, P < 0.001. h Transwell assays revealed that overexpression of miR-556-5p restrained migration and invasion abilities of DLD-1 and HCT116 cells, ***, P < 0.001. i–k Rescue experiment showed that miR-556-5p could partially reverse the oncogenic function of circHERC4 in proliferation, migration and invasion, **, P < 0.01, ***, P < 0.001

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