Fig. 2
BCL-XL degradation with DT2216 enhances the antitumor efficacy of sotorasib in KRASG12C-mutated xenograft models. a–c Tumor volume changes in H358 (a), MIA PaCa-2 (b) and SW837 (c) xenografts after they were treated with vehicle, DT2216 (DT, 15 mg/kg, every four days [q4d], i.p.), sotorasib (Sot, 10 mg/kg, 5 days a week, p.o.) or a combination of the two (Combo). Data are presented as mean ± SEM (n = 7–8 mice at the start of treatment). Statistical significances in a–c were determined by two-sided Student’s t-test at day-41, day-25 and day-25 in H358, MIA PaCa-2 and SW837 xenografts, respectively. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. d–f Changes in tumor volumes of individual mice in H358 (d), MIA PaCa-2 (e) and SW837 (f) xenografts at the end of treatment calculated as: tumor volume at the end of treatment − baseline tumor volume. g–i Densitometric analysis showing BCL-XL protein levels as determined by immunoblotting in H358 (g), MIA PaCa-2 (h) and SW837 (i) xenograft tumors at the end of treatments as in a–c (n = 4 mice per group). The representative immunoblots are shown in Additional file 1: Fig. 12a