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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Reductive TCA cycle catalyzed by wild-type IDH2 promotes acute myeloid leukemia and is a metabolic vulnerability for potential targeted therapy

Fig. 2

Impact of IDH2 knockdown on AML growth in vivo. a Outline of in vivo study design using AML cell models (U937 and ML-1) with shRNA-Ctrl and shIDH2#1 or shIDH2#2. b–d Tumor growth in athymic nude mice inoculated with U937 cells harboring shRNA-Ctrl, shIDH2#1 or shIDH2#2 (n = 5 per group, mean ± SEM). Tumor sizes were measured every two days. At the end of the experiment, tumors were isolated, photographed and weighted. e–g Tumor growth in athymic nude mice inoculated with ML-1 cells harboring shRNA-Ctrl, shIDH2#1 or shIDH2#2 (n = 7 per group, mean ± SD). Tumor sizes were measured every two days. Tumors were photographed and weighted at the end of the experiment. The star * in f indicates no tumor formation in this mouse. h Representative Western blotting of IDH2 protein expression in tumor tissues isolated from mice inoculated with AML cells harboring shRNA-Ctrl or IDH2 shRNA as indicated. i Left panel: representative images of Ki-67 IHC staining of tumor tissues from U937 xenografts with shRNA-Ctrl or IDH2 shRNA as indicated. The scale bars represent 50 μm; Right panel: apoptotic cells in tumor tissues of U937 xenografts with shRNA-Ctrl or IDH2 shRNA detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The scale bars represent 100 μm. ***p < 0.001

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