Fig. 4

Biodistribution and tumor targeting of F-AgNPs in vivo. a, b The dynamics of DiR signals over time within tumor tissue after systemically delivering DiR-labeled F-AgNPs. Data are represented as mean ± s.e.m. n = 3. Student’s t test was used for statistical analysis. ns not significant. c Evaluation of in vivo antigen modification effects through observing the fluorescence images of MKN45 subcutaneous tumors sections after intravenous injection of F-AgNPs or P-NPs (FAM-labeled). Tumors were collected 2 h, 8 h and 24 h after delivery. Nucleus (DAPI, blue); antigen peptide (FAM, green); vessal (Cy5, red). Scale bar, 100 μm. d, e The dynamics of DiR signals over time in tumor and organs after intraperitoneal injection of DiR-labeled F-AgNPs. Data are represented as mean ± s.e.m. n = 4. Student’s t test was used for statistical analysis. ns not significant; *p < 0.05