Fig. 3

The role of T cell exhaustion markers in the overall survival of cholangiocarcinoma. A-R and V, Kaplan–Meier curves of overall survival (OS) of patients whose tumors are grouped by higher density versus lower density of exhausted T cell subtypes as indicated. Log rank tests were performed with p values as indicated. The correlation of the density of CD4⁺PD-1+ T-cells (A–C), CD8⁺PD-1⁺ T-cells (D–F), CD8⁺EOMES⁺PD-1⁺ T-cells (G–I), CD8⁺EOMES⁻PD-1⁺ T-cells (J–L), CD8⁺EOMES⁺PD-1⁻ T-cells (M–O), and CD8⁺EOMES⁻PD-1⁻ T-cells (P–R) with OS. (S–U) Percentages of CD8⁺ T cells among CD45⁺ T cells were shown in the pie graphs; and CD8⁺ T cells were further divided according to the status of PD-1 and EOMES as shown in the pie graphs. V Kaplan–Meier curves of overall survival of patients whose tumors are grouped by higher density versus lower density of CD3⁺CD8⁺EOMES⁻PD-1⁻ T cells in the tumor areas (left panel) and in the peripheries of the tumors (right panel), respectively. DCC, distal cholangiocarcinoma; HC, hilar cholangiocarcinoma; ICC, intrahepatic cholangiocarcinoma