Fig. 4

Compound 1 reduced ENL, but not other SEC proteins in MLL1-r leukemia cells. a, b Upon compound treatment at the specified concentrations for 4 days, levels of ENL and other related proteins in the a nucleus and b cytoplasm of MV4;11 cells, showing 1 only significantly reduced ENL; c–g ChIP-qPCR results showing the enrichment of c ENL, d AF9, e AFF4, f cyclin-T1 and g H3K79me2 in the gene promoters of Myc and HoxA9. Treatment with compound 1 (500 nM) only significantly reduced the binding of ENL to these gene promoters. (*p < 0.05)