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Fig. 7 | Journal of Hematology & Oncology

Fig. 7

From: A proteolysis-targeting chimera molecule selectively degrades ENL and inhibits malignant gene expression and tumor growth

Fig. 7

Compound 1 degraded mutant ENL (mENL) and suppressed its mediated gene transcription. a Cellular levels of mutant/wild-type (WT) ENL and β-actin detected by (left panel) FLAG or (right panel) ENL antibody, upon transfection with increasing amounts of a mENL-containing plasmid for 4 h followed by 24 h incubation, showing dose-dependent expression of mENL. Endogenous WT ENL can also be detected and included in quantification (right panel); b, c Levels of mutant/WT ENL detected by a FLAG or ENL antibody, upon transfection with b 0.1 or c 0.2 μg of the plasmid for 4 h followed by 24 h treatment with compound 1, showing the compound can dose-dependently degrade or deplete both mutant and WT ENL; d Transfection with 0.04 μg of the plasmid for 4 h followed by 24 h incubation upregulated expression of HoxA11 (left) and HoxA13 (right), and treatment with 1 during the incubation inhibited such gene overexpression (*p < 0.05)

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