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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Universal immunotherapeutic strategy for hepatocellular carcinoma with exosome vaccines that engage adaptive and innate immune responses

Fig. 1

Evaluation of DEXP&A2&N’s tumor-targeting, DC-recruiting and DC-activating ability in orthotopic mCherry-expressing HCC mice. a Schematic illustration for designer DEX vaccine-DEXP&A2&N. P-P47; A2-AFP212; N-N1ND. DEX refers to DC-derived exosomes. b Flow cytometric analysis to assess the simultaneous binding efficiency of three moieties on DEX (n = 4). Diagram for dosing regimen (c) and tissue distribution and quantitative analysis of labeled DEXP&A2&N (d) in day-14 orthotopic HCC mice bearing mCherry-expressing tumors. DiR-labeled DEXA2&N (n = 9), DEXP&A2&N (n = 9) (80 μg/mouse) or PBS (n = 4) were injected into day-14 orthotopic HCC mice bearing mCherry-expressing tumors intravenously, and tissues were harvested 2 h after injection (one-way ANOVA post hoc Student–Newman–Keuls test was used except for liver and tumor in which one-way ANOVA on ranks was used). mLN-mesenteric lymph node; iLN-inguinal lymph node; Tu-tumor. Flow cytometric and quantitative analysis of CD11c+ DCs in tumor-infiltrating lymphocytes (TILs) from HCC mice bearing mCherry-expressing tumors (n = 13; one-way ANOVA post hoc Student–Newman–Keuls test). Flow cytometric (f) and quantitative analysis (g) of CD103+CD11c+ (one-way ANOVA on ranks) and CD8α+ CD11c+ DCs (one-way ANOVA post hoc Student–Newman–Keuls test) in TILs from HCC mice (n = 9). h Flow cytometric and quantitative analysis of surface protein markers on CD11c+ DCs from tumors of orthotopic HCC mice treated with DEXP&A2 or DEXP&A2&N (n = 5; one-way ANOVA post hoc Student–Newman–Keuls test). i Flow cytometric and quantitative analysis of surface protein markers on CD103+CD11c+ and CD8α+CD11c+ DCs from tumors of orthotopic HCC mice treated with DEXP&A2 or DEXP&A2&N (n = 5; one-way ANOVA post hoc Student–Newman–Keuls test). *p < 0.05, **p < 0.001; n.s, not significant

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