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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: A novel tRNA-derived fragment AS-tDR-007333 promotes the malignancy of NSCLC via the HSPB1/MED29 and ELK4/MED29 axes

Fig. 5

AS-tDR-007333 directly binds to and interacts with HSPB1 in NSCLC cells. A Silver SDS-PAGE gel image revealed proteins immunoprecipitated by AS-tDR-007333 and its antisense RNA in PC9 cells. B AS-tDR-007333 binding proteins with top matching scores identified by mass spectrometry. C RIP assay followed by qRT-PCR analysis confirmed that AS-tDR-007333 specifically bound to HSPB1 protein in PC9 cells. D Predicted 3D structure of the AS-tDR-007333–HSPB1complex. E The expression levels of HSPB1 protein in NSCLC cells were higher than that in BEAS-2B cells. F The expression levels of HSPB1 gene in NSCLC cells were significantly higher than that in BEAS-2B cells. G si-HSPB1 significantly repressed cell proliferation of PC9 cells. H Inhibition of HSPB1 gene expression decreased cell proliferation rate of A549 cells. I Rescue assays showed that co-transfection of AS-tDR-007333 and si-HSPB1 suppressed the promoting ability of AS-tDR-007333 on cell proliferation of PC9 cells. J Co-transfection of AS-tDR-007333 and si-HSPB1 decreased the capacity of AS-tDR-007333 for enhancing cell proliferation rate in A549 cells. *P < 0.05; **P < 0.01; ***P < 0.001

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