Fig. 1

LncRNA-PACERR expression in TAMs is activated in PDAC tissues and is associated with poor prognosis. A Schematic illustration of qRT-PCR forty-six sample preparation (n = 46). Tumour-associated macrophages (TAMs) from forty-six PDAC tissue samples were enriched with CD163 positive selection. M1 macrophages in normal tissue-resident macrophages (M1-NTRMs) from PDAC adjacent normal tissue samples of the same patient were enriched with CD80 positive selection. B Purity of CD163⁺ cells and CD80⁺ by CD163 and CD80 microbeads sorting. C Expression of LncRNA-PACERR in 46 pairs of TAMs and M1-NTRMs from PDAC. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. D Colocalization of LncRNA-PACERR (green) and CD163/CD80 (red) in 110 clinical samples of pancreatic ductal adenocarcinoma (PDAC) as shown by fluorescence microscopy. DAPI staining (blue) shows the nuclei (DNA). Scar bar: 50 μm. E Staining intensity of LncRNA-PACERR, CD163 and DAPI on the immunofluorescence from TMAs of 110 PDAC patients. Green represents LncRNA-PACERR. Red represents CD163. Blue represents DAPI. F Kaplan–Meier survival curve presenting the overall survival of 110 PDAC patients, grouped according to the extent of LncRNA-PACERR⁺ TAMs infiltration