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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: LncRNA-PACERR induces pro-tumour macrophages via interacting with miR-671-3p and m6A-reader IGF2BP2 in pancreatic ductal adenocarcinoma

Fig. 2

Knockdown of LncRNA-PACERR hinders the M2 polarization and pro-tumour functions of THP-1-derived TAMs in vitro. A qPCR analysis of the relative expression of M2 markers (Arginase-1, CD163, TGFβ, CD206, IL-10 and IL-6) and M1 marker (CD80, IL-1β) in THP-1-derived TAMs after LncRNA-PACERR knockdown. THP-1 cells were treated with PMA and co-cultured with PANC-1 cells for two days. Data are shown as the results from three independent experiments. B Flow cytometric analysis of the expression of M2 markers (CD163 and CD206) in THP-1-derived TAMs after LncRNA-PACERR knockdown. THP-1 cells were treated with PMA and co-cultured with PANC-1 cells for two days. Data are shown as the results from two independent experiments. C–E Proliferation (C), migration (D) and invasion (E) capacity of PATU-8988 or PANC-1 cells co-cultured with THP-1-derived TAMs (shNC/ shPACERR). shNC means that cells were transfected in negative control plasmids. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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