Fig. 5

MiR-671-3p directly binds to the 3′UTR of KLF12 and regulates the KLF12/AKT/c-myc axis. A Predicted target genes of miR-671-3p using Starbase, miRWalk, miRbase, RNA22 and the two overlapping target genes (NMT-1 and KLF12). B Expression of KLF12 in 46 pairs of TAMs and M1-NTRMs from PDAC patients. C Correlation analysis between miR-671-3p and KLF12 using data from 46 pairs of PDAC patients. D mRNA expression of KLF12 in THP-1 derived TAMs (co-cultured with PATU-8988 or PANC-1 cells) transfected with empty control or miR-671-3p mimics or inhibitor. E, F Dual luciferase activity in HEK-293 T cells co-transfected with the KLF12 wild-type or mutant sequence (E) and miR-671-3p mimics. G Correlation analysis between LncRNA-PACERR and KLF12 in TAMs from 46 PDAC patients. H mRNA expression of KLF12 in THP-1 derived TAMs co-transfected with the LncRNA-PACERR knocked down vector and miR-671-3p inhibitor. I Protein levels of KLF12 in THP-1 derived TAMs (co-cultured with PATU-8988 or PANC-1 cells). J Dual luciferase activity in HEK-293 T cells co-transfected with LncRNA-PACERR overexpression vector and miR-671-3p mimics. K, L Potein expression of PI3K/KLF12/AKT/c-myc axis in THP-1 derived TAMs (co-cultured with PANC-1 (K) or PATU-8988 cells (L)). M LncRNA-PACERR expression was evaluated by qRT-PCR in THP-1 derived TAMs with indicated treatment. The data are presented as the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001