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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Post-transplant cyclophosphamide alters immune signatures and leads to impaired T cell reconstitution in allogeneic hematopoietic stem cell transplant

Fig. 1

PTCy significantly impacts the T cell subsets by increasing Treg and reducing naïve T cells. The frequencies of conventional CD4+ T cells (CD4+ Tcon), CD8+ T cells and regulatory T cells (Treg) subsets in total CD3+ T cells A and their absolute numbers in peripheral blood per μL B are displayed as box-and-whisker plots. C Representative flow-cytometry showing the gating strategy to define Treg subsets based on the expression of CD45RA and FOXP3 (left); the identification of resting Treg (CD45RA+FoxP3int) and activated Treg (CD45RAFoxP3high) subsets is shown in the right plot. D The frequencies of Treg subsets in total CD3+ T cells are displayed as box-and-whisker plots. E Representative gating strategy was used to define the subpopulation of CD4+ Tcon and CD8+ T cells based on expression of CD45RA and CCR7. T cells were divided into 4 subgroups, naïve cells (TN), central memory (TCM), effector memory (TEM) and terminally differentiated effector memory (TMERA). F Summarized columns showing the component of T cell subsets of PTCy (P) versus non-PTCy (NP) group at designated timepoints. The data are presented as mean ± SEM. G The absolute cell number of each T cell subset in peripheral blood per μL. Each dot represents the corresponding value from one single patient. Asterisks denote statistical differences comparing the two groups at different timepoints; P values were obtained by the Wilcoxon-rank sum test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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