Fig. 1

Mammary adipocytes protect breast cancer cells from ferroptosis. A Lipid peroxidation levels in co-culture and control group of MDA-MB-231 and BT-549 cells treated with DMSO or SAS (2 mM). B Quantitative analysis of lipid peroxidation levels of co-culture and control cells treated with DMSO or SAS (2 mM). C Fluorescence microscopy images of co-culture and control cells treated with DMSO or SAS or SAS in combination with ferrostatin-1 (5 μM). D TEM images of co-culture and control cells treated with SAS. E Cell viability in co-culture and control group of MDA-MB-231 and BT-549 cells treated with indicated concentrations of SAS. F Representative images of clonogenic assay in co-culture or control group of cells subjected to the indicated treatments. G Quantification of clonogenic survival fractions in cells subjected to the indicated treatments