Fig. 2

Adipocytes protect breast cancer cells from ferroptosis through oleic acid in the presence of ACSL3. A Lipid peroxidation levels in MDA-MB-231 and BT-549 cells co-cultured with exogenous fatty metabolites oleic acid (OA, 500 μM) and treated with SAS or DMSO. B Quantitative analysis of lipid peroxidation levels in cells co-cultured with exogenous fatty metabolites OA (500 μM), stearic acid (25 μM), glycerol (500 μM) and DMSO, then treated with SAS or DMSO. C The mRNA and protein expression levels of ACSL3 confirmed by qRT-PCR and western blotting in MDA-MB-231 and BT-549 cells expressing control vector (shNC) or sh-ACSL3. D Lipid peroxidation levels in shACSL3 and shNC MDA-MB-231 and BT-549 cells co-cultured with adipocytes or OA and treated with DMSO or SAS. E Quantitative analysis of lipid peroxidation levels in shACSL3 and shNC cells co-cultured with adipocytes or OA and treated with DMSO or SAS. F Schematic diagram showing nude mice bearing MDA-MB-231 xenografts at fat pad (FP) of left groin and right back subcutaneously (SC). G Body weights of nude mice bearing xenografts treated with SAS, P values were calculated by 2-tailed unpaired Student’s t-test. H Volume of MDA-MB-231 xenografts at FP or SC treated with normal saline (control) or SAS at different time points. P values determined using 2-way ANOVA