Fig. 2
Enhanced tumor formation and growth is associated with a higher IR/IGF1R ratio resulting from TRIP-Br1 expression. A, B MCF7WT-TRIP-Br1 and MCF7KD-TRIP-Br1 cells were injected into nude mice. The tumors were collected and photographed. Scale bar, 1 cm. Tumor weight was measured after mice resection (n > 6). C, D Tumor was collected and subjected to western blotting. The relative IR/IGF1R ratio is presented as the mean ± SD (n > 6; ***p < 0.005). E, F Tissue samples from the adipocytes were collected from 5-week-old insulin-producing mice (control) or insulin-deficient mice (IDM). The tissues were used to assess the levels of TRIP-Br1, IR, and IGF1R by western blot analysis, in which insulin and glucagon were used as controls (n > 3). The relative IR/IGF1R ratio is shown. The quantification results are presented as the mean ± SD (*p < 0.05; **p < 0.01; ***p < 0.005). G, H Representative images of IHC analysis show the expression levels of TRIP-Br1, IR, and IGF1R in the adipocytes of control or IDM groups. The expression levels of TRIP-Br1, IR, and IGF1R are presented as the mean ± SD (n > 3). The relative IR/IGF1R ratio is shown. (*p < 0.05; ***p < 0.005). I The correlation of TRIP-Br1 and IR/IGF1R ratio in four subtypes of breast cancer. Each dot represents a single cell. J Summary model shows the regulation of the IR/IGF1R ratio by TRIP-Br1 in breast cancer cells