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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Long noncoding RNA Smyca coactivates TGF-β/Smad and Myc pathways to drive tumor progression

Fig. 1

Smyca promotes EMT, migration, invasion and stemness. (A) Morphological assessment of MDA-MB-231 cells stably expressing four different Smyca shRNAs. Bar, 100 μm. Cell length/width ratios and Smyca expression levels (normalized to the control group) are shown in the middle and right panels, respectively. Data are mean ± SD, n = 30 (middle) or 3 (right). P values are determined by one-way ANOVA with Tukey’s post hoc test, *** P < 0.001. (B, G) Western blot analysis of EMT markers in MDA-MB-231 cells stably expressing Smyca shRNAs (B) or M10 cells stably overexpressing Smyca (G). The amounts of each protein in relation to the control cells are indicated under the bands. (C, H) Migration and invasion assays of MDA-MB-231 cells stably expressing Smyca shRNAs (C) or M10 cells stably overexpressing Smyca (H). Data are mean ± SD from three independent experiments. P values are determined by one-way ANOVA with Tukey’s post hoc test (C) or unpaired t test (H), *P < 0.05, **P < 0.01, *** P < 0.001. (D, I) Flow cytometry analysis of the expression of breast cancer stem cell markers in MDA-MB-231 cells stably expressing Smyca shRNAs (D) or M10 cells stably overexpressing Smyca (I). Stem cell populations are marked by blue and the percentages are indicated. (E, J) Sphere formation assay of MDA-MB-231 cells stably expressing Smyca shRNAs (E) or M10 cells stably overexpressing Smyca (J). Solid and dashed lines are derived from means and standard deviations, respectively. Data are mean ± SD from three independent experiments. P values are determined by Chi-square test. (F) Morphological assessment of M10 cells stably overexpressing Smyca. Bar, 100 μm. Cell length/width ratios and Smyca expression levels (normalized to the control group) are shown in the middle and right panels, respectively. Data are mean ± SD, n = 30 (middle) or 3 (right). P values are determined by unpaired t test, *** P < 0.001

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