Fig. 2

Overexpression of GPD1 leads to bladder cancer cell apoptosis and inhibits tumor growth. A and B Western blotting to determine GPD1 overexpression in 5637 and T24 cells. C and D CCK-8 assay to evaluate the proliferation of GPD1-overexpressing 5637 and T24 cells in vitro. E and F Colony formation of 5637 cells (control or GPD1 overexpression) and T24 cells (control or GPD1 overexpression). The number of colonies formed was counted by ImageJ. G and H Flow cytometry analysis with Annexin V-PI staining was performed to evaluate the percentage of apoptotic cells in GPD1-overexpressing 5637 and T24 cells. I Control 5637 cells or GPD1-overexpressing 5637 cells were implanted in BALB/c Nude mice. Tumors were resected and measured 4 weeks later. J Control T24 cells or GPD1-overexpressing T24 cells were implanted in BALB/c Nude mice. Tumors were resected and measured 4 weeks later. K and L H&E staining and TUNEL assay of 5637 tumor tissue or T24 tumor tissue from the control or GPD1 overexpression group