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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Allosteric activation of the metabolic enzyme GPD1 inhibits bladder cancer growth via the lysoPC-PAFR-TRPV2 axis

Fig. 5

GPD1 promotes TRPV2 upregulation via lysoPC-PAFR axis. A Untargeted metabolomics of control 5637 cells, GPD1-OE 5637 cells and K120A GPD1-OE 5637 cells under positive ion mode and negative ion mode. Venn diagram showing the overlap of differential metabolites between the three groups. KEGG was used to explore relevant metabolic pathways. B and C Flow cytometry analysis of apoptosis in 5637 or T24 cells treated with lysoPC. D and E Flow cytometry was performed to detect TRPV2 expression on the membranes of 5637 and T24 cells treated with lysoPC. F and G Flow cytometry was performed to detect intracellular Ca2+ levels in 5637 cells and T24 cells treated with lysoPC. H and I Expression of lysoPC receptors in 5637 cells and T24 cells by RT-qPCR. J and K Flow cytometry analysis of apoptosis in 5637 or T24 cells treated with lysoPC in the presence of the PAFR inhibitor ginkgolide B. L and M Flow cytometry was performed to detect TRPV2 expression on the membranes of 5637 and T24 cells treated with lysoPC in the presence of ginkgolide B. N Flow cytometry analysis of apoptosis in 5637 cells or T24 cells treated with G3P/NAD+ in the presence of ginkgolide B. O Flow cytometry was performed to detect TRPV2 expression on the membranes of 5637 and T24 cells treated with G3P/NAD+ in the presence of ginkgolide B

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