Fig. 1
From: Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients
Arterial thrombi from COVID-19 + patients contain SARS-CoV-2 SP but not N protein. Panel 1. Immunostaining positive for SARS-CoV-2 Spike protein (SP) (arrows) in representative thrombotic material from COVID-19 + patients, retrieved from cerebral (A) and coronary (C) arteries. Immunohistochemistry for nucleocapsid protein (NP) was negative in the same samples (B-D). Panel 2. Representative immunohistochemical staining positive for SP (E) and NP (F) (arrows) in the lung of a patient affected by COVID-19 (positive control). Representative immunostaining negative for SP (G) and NP (H) in a thrombus retrieved from the middle cerebral artery of a patient not affected by COVID-19 (negative control). Original magnification 20X. Panel 3. Double immunofluorescence of thrombotic material retrieved from COVID-19 and non-COVID-19 patients’ cerebral arteries. In the COVID-19 thrombus, platelets are co-stained with anti-CD61 (red—L,P) and anti-SARS-CoV-2 spike protein (SP) antibodies (green—M,Q), emitting yellow signals in the merged panel (N), while in the control (non-COVID thrombus) only the red CD61 signal is observed (R)