Fig. 1

Cellular responses to TRT and EBRT. a A431/CCKBR cells were treated with [¹⁷⁷Lu]Lu-PP-F11N or exposed to EBRT and the generated tryptic peptides and phosphopeptide enriched samples were subjected to mass spectrometry analysis. b Charts display phosphopeptide (upper panels) and protein (lower panels) abundance changes shown as log2 transformed fold change (FC). Red dots represent phosphopeptides or proteins with significantly altered abundance. FDR < 0.05. c Hierarchical clustering of identified changes in phosphopeptide and protein abundance. d Number of phosphopeptides and protein abundance changes. Arrows indicate up-regulated (purple) or down-regulated (blue) phosphorylation or protein levels. e MS2-based quantification of abundance changes was shown as Log2 transformed radiation/control ratios for CYR61, GAPDH, ERK2 and P53 protein levels and phosphorylation of ERK, P53 and JUN after exposure to [¹⁷⁷Lu]Lu-PP-F11N and EBRT in A431/CCKBR cells. f, g Expression and phosphorylation levels of the same proteins were validated by quantitative WB analysis on total protein lysates isolated from untreated (control) and [¹⁷⁷Lu]Lu-PP-F11N- or EBRT-treated cells. Each treatment was performed in triplicate (Exp 1–3). Quantification of signal intensities (in f) is shown as Log2 transformed radiation/control ratios as described above. *P < 0.05, **P < 0.01, ***P < 0.001. h WB analysis for CYR61 and P53 phosphorylation in total protein lysates isolated from A431/CCKBR cells untreated (control) and treated with 1, 2, 4, 6 and 8 Gy at indicated time points. Blot was re-probed with antibody against GAPDH and total P53