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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Hypoxia-induced exosomal circPDK1 promotes pancreatic cancer glycolysis via c-myc activation by modulating miR-628-3p/BPTF axis and degrading BIN1

Fig. 3

Hypoxia-derived exosomal circPDK1 promotes PC cells proliferation and metastasis in vitro and in vivo. A MIA PaCa-2 and PANC-1 cells were cultured in 6-well plates after treated with indicated exosomes. Scale bar = 1000 mm. B The viabilities of PC cells were detected by CCK-8 assays after treated with indicated exosomes. C The EdU assay was used to assess the cell proliferative potential of PC cells after treated with indicated exosomes. Scale bar = 50 μm. D Transwell migration assay of MIA PaCa-2 and PANC-1 after treatment with the indicated exosomes. Scale bar = 50 μm. E The expression of metastasis-related proteins was evaluated by Western blotting after treated with indicated exosomes. F Images of subcutaneous tumors. G The weight of subcutaneous tumors in each group. H Mice were injected through tail vein with indicated exosomes and the tumor volume was calculated every three days. I The body weight of mice in each group. J Representative photographs of the whole lung tissues and HE staining of lung metastatic nodules. K Representative photographs of PCNA, E-cadherin and vimentin IHC staining in subcutaneous tumors. Scale bar = 50 μm. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance

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