Fig. 2

Murine CXCL13 is increased in the blood and BM of MM-inoculated mice. A Evaluation of cytokine levels in the serum of MM-inoculated (pooled from 3 mice) and non-injected control (pooled from 3 mice) animals using cytokine array. B Levels of mCXCL13 in the BM of MM-inoculated (n = 6) and control mice (n = 3), evaluated by qRT-PCR. Data are presented as the mean of triplicates ± STDEV (**p < 0.01). C Immunohistochemistry for mCXCL13 and human CXCL13. D, E RPMI8226-CXCR4-GFP cells were injected i.v. into NSG mice. Mice were either untreated (control group, n = 4) or treated with subcutaneous injections of bortezomib (1 mg/kg, twice a week) (n = 4). D Tumor burden (%GFP + cells) in BM and serum levels of murine CXCL13 were evaluated on day 24. E Co-expression of human β2-microglobulin and murine Cxcl13 in the BM of MM-inoculated mice, evaluated by qRT-PCR