Fig. 3

Interaction between MM cells and macrophages up-regulates CXCL13 in both cell populations. A CXCL13 mRNA expression in human MM cell lines, primary BMSC, and peripheral-blood generated MΦ, evaluated by qRT-PCR. Data are presented as the mean of triplicates ± STDEV (**p < 0.01). B Peripheral-blood-derived MΦ were cultured in the absence or presence of MM cell lines RPMI8226 or CAG (direct co-culture) for 48 h. Levels of secreted CXCL13 in the culture medium were evaluated by ELISA. Data are presented as the mean of triplicates ± STDEV (**p < 0.01). C CXCL13 mRNA levels in MM cells RPMI8226 and CAG, cultured in the absence or presence of peripheral-blood derived MΦ, either in direct contact co-culture or separated by 0.4 µm membrane, evaluated by qRT-PCR. D CXCL13 mRNA levels in peripheral-blood generated MΦ, cultured in the absence or presence of RPMI8226 and CAG cells, separated by a 0.4 µm membrane, evaluated by qRT-PCR. Data are presented as the mean of triplicates ± STDEV (**p < 0.01)