Fig. 5

Reciprocal regulation of CXCL13 in RANKL in BMSC and Mф. Induction of osteoclast generation by CXCL13. RPMI8226 cells, primary BMSC or peripheral blood-generated macrophages were A treated with RANKL (100 ng/ml) for 48 h and mRNA level of CXCL13 was tested by qPCR, and B treated with CXCL13 (500 ng/ml) for 48 h and mRNA levels of RANKL was tested by qPCR. Data are presented as the mean of triplicates ± STDEV (**p < 0.01). C Human peripheral blood mononuclear cells from healthy donors were grown in the presence of recombinant human M-CSF (100 ng/ml) and RANKL (100 ng/ml) or CXCL13 (500 ng/ml) −/+ anti-CXCL13 (10 µg/ml) for 2 weeks. TRAP staining was performed and multinucleated TRAP+ cells were enumerated