Fig. 1

ERRα expression and OXPHOS pathway higher in AML cells. A ERRα expression comparison between hematopoietic stem or progenitor cells in 7 healthy donors and leukemic sub-population in 21 AML patients. Subtype information as well as CD34 status of individual sample is available in GSE63270; Hematopoietic stem cell (HSC), Multipotent progenitors (MPP), Common Myeloid Progenitor (CMP), Lymphoid-primed multipotent progenitor (LMPP), Granulocyte-erythroid progenitor (GMP), Megakaryocyte-erythroid progenitors (MEP). B ERRα expression comparison between healthy controls and AML patients in two independent cohorts (E-MTAB-220 and GSE9476). C ERRα expression comparison between AML cell lines (n = 32) and LM22 immune reference cells (n = 195). A–C P values were calculated by two-tailed t test. D Immunohistochemistric analysis of ERRα protein expression in bone marrow samples from three non-leukemic donors and four AML patients. E KM plots showing survival probability differences among patients stratified by mean and standard deviation of ERRα expression into low, medium, and high groups. P values were calculated by log-rank test (LRT). F Schematic definition of ERRα target genes from integration of ChiP and RNA-Seq data. G HALLMARK genes set significantly enriched (FDR < 0.01) within ERRα + genes in AML cell lines. H Clustering 5162 cells into aneuploid, and diploid cells based on copy number alterations determined by CopyKat (left). ERRα expression among the 5162 cells (right). I Comparison of ERRα expression between aneuploid and diploid cells. J Comparison of OXPHOS pathway activity between individual aneuploid and diploid cells. K Comparison of OXPHOS pathway activity between ERRα expressing aneuploid cells and other aneuploid cells. (I–K) P values were measured by two-tailed Wilcoxon rank sum test. Each dot in the figure represents a single cell